Journal of Advanced Pharmacy Education & Research
 
 
Year : 2014   |  Volume : 4   |  Issue : 1   |  Page : 101-105  

Quantitative analysis method development and validation for Irbesartan in bulk drug by Ultraviolet spectroscopy

Amit Asati, Anita Shinde1, Suman Malik, K. C. Asati

Correspondence Address:1Department of Chemistry I. E. H. E, Bhopal, India 2Department of Chemistry, Sadhu Vaswani College Bairagarh, Bhopal, India

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-4040.197331

Abstract  

A simple, rapid and precise ultraviolet spectroscopy method was developed and validated for quantitative analysis of irbesartan in bulk drug. It is an angiotensin II receptor (AT1 subtype) antagonist. It is used for the treatment of hypertension. It shows maximum absorbance at 246 nm. Beer Lambert’s was obeyed in the concentration range of in the range of 5-30 µg/ml. The method was successfully validated in accordance to USP pharmacopeia and ICH guideline for accuracy, precision, rang and linearity. The linear regression analysis data for calibration plots showed good linear relationship and obtain correlation factor is greater than 0.999 for irbesartan. The % Recovery/Accuracy was within the range between 98% and 102%. The percentage RSD for precision was found to be less than 2%. Thus method was successfully applied for routine analysis of irbesartan in bulk samples.

Keywords: Accuracy, ICH guideline, Irbesartan, Precision, Validation, ultraviolet spectroscopy USP pharmacopeia.

How to cite this article:
Amit Asati2*, Anita Shinde1,

Introduction   Top

Materials and methods   Top

Results Discussion   Top

Conclusion   Top

Acknowledgement   Top

References   Top

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