Journal of Advanced Pharmacy Education & Research
 
 
Year : 2014   |  Volume : 4   |  Issue : 2   |  Page : 247-258  

Immunosuppressive effect of Eupalitin-3-O-?-D-Galactoside in Conconavalin-A induced KOI CARP (Cyprinus Carpio)

K. A. Fathima, C. S. Parameswari

Correspondence Address:1Associate Professor in Biochemistry, Bharathiwomen’s college, Chennai-108, Tamilnadu, India. 2 Associate professor in Biochemistry, Bharathiwomen’s college, Chennai- 108,Tamilnadu,India.

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-4040.197331

Abstract  

Eupalitin-3-O-ß-D-galactoside a bioflavonoid isolated from Boerhaavia diffusa has been reported to possess immunosuppressive activity.To investigate the potential therapeutic effect of Eupalitin-3-O-ß-D-galactoside,Koi carp a fish model was induced with Conconavalin-A and Eupalitin-3-O-ß-D-galactoside was injected intramuscularly 48 hrs after Conconavalin-A administration.The results revealed that Eupalitin-3-O-ß-D-galactoside attenuated the activity of serum lysozyme and myeloperoxidase in serum and expression of TNF-a expression in head kidney tissues of koi carp.Western blotting results revealed that NFkB and p38 MAPK expression were downregulated after Eupalitin-3-O-ß-Dgalactoside treatment.These results suggested the anti-inflammatory effect of Eupalitin-3-O-ß-D-galactoside in Conconavalin-A induced koi carp possibly through inhibition of NFkB and p38 MAPK that mediates the expression of pro inflammatory cytokine TNF-a.

Keywords: Eupalitin-3-O-ß-D-galactoside, Conconavalin-A (ConA), Tumor necrosis factor (TNF-a), Nuclear factor-kappaB (NF-?B), P38 MAPKinase

How to cite this article:
: *K. A. Fathima1, C. S.

Introduction   Top

Materials and methods   Top

Results Discussion   Top

Conclusion   Top

Acknowledgement   Top

References   Top

Figures

Immunosuppressive effect of Eupalitin-3-O-?-D-Galactoside in Conconavalin-A induced KOI CARP (Cyprinus Carpio)

K. A. Fathima, C. S. Parameswari

Correspondence Address:1Associate Professor in Biochemistry, Bharathiwomen’s college, Chennai-108, Tamilnadu, India. 2 Associate professor in Biochemistry, Bharathiwomen’s college, Chennai- 108,Tamilnadu,India.

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-4040.197331

Abstract  

Eupalitin-3-O-ß-D-galactoside a bioflavonoid isolated from Boerhaavia diffusa has been reported to possess immunosuppressive activity.To investigate the potential therapeutic effect of Eupalitin-3-O-ß-D-galactoside,Koi carp a fish model was induced with Conconavalin-A and Eupalitin-3-O-ß-D-galactoside was injected intramuscularly 48 hrs after Conconavalin-A administration.The results revealed that Eupalitin-3-O-ß-D-galactoside attenuated the activity of serum lysozyme and myeloperoxidase in serum and expression of TNF-a expression in head kidney tissues of koi carp.Western blotting results revealed that NFkB and p38 MAPK expression were downregulated after Eupalitin-3-O-ß-Dgalactoside treatment.These results suggested the anti-inflammatory effect of Eupalitin-3-O-ß-D-galactoside in Conconavalin-A induced koi carp possibly through inhibition of NFkB and p38 MAPK that mediates the expression of pro inflammatory cytokine TNF-a.

Keywords: Eupalitin-3-O-ß-D-galactoside, Conconavalin-A (ConA), Tumor necrosis factor (TNF-a), Nuclear factor-kappaB (NF-?B), P38 MAPKinase

How to cite this article:
: K. A. Fathima*

Introduction   Top

Materials and methods   Top

Results Discussion   Top

Conclusion   Top

Acknowledgement   Top

References   Top

Figures

Immunosuppressive effect of Eupalitin-3-O-?-D-Galactoside in Conconavalin-A induced KOI CARP (Cyprinus Carpio)

K. A. Fathima, C. S. Parameswari

Correspondence Address:1Associate Professor in Biochemistry, Bharathiwomen’s college, Chennai-108, Tamilnadu, India. 2 Associate professor in Biochemistry, Bharathiwomen’s college, Chennai- 108,Tamilnadu,India.

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-4040.197331

Abstract  

Eupalitin-3-O-ß-D-galactoside a bioflavonoid isolated from Boerhaavia diffusa has been reported to possess immunosuppressive activity.To investigate the potential therapeutic effect of Eupalitin-3-O-ß-D-galactoside,Koi carp a fish model was induced with Conconavalin-A and Eupalitin-3-O-ß-D-galactoside was injected intramuscularly 48 hrs after Conconavalin-A administration.The results revealed that Eupalitin-3-O-ß-D-galactoside attenuated the activity of serum lysozyme and myeloperoxidase in serum and expression of TNF-a expression in head kidney tissues of koi carp.Western blotting results revealed that NFkB and p38 MAPK expression were downregulated after Eupalitin-3-O-ß-Dgalactoside treatment.These results suggested the anti-inflammatory effect of Eupalitin-3-O-ß-D-galactoside in Conconavalin-A induced koi carp possibly through inhibition of NFkB and p38 MAPK that mediates the expression of pro inflammatory cytokine TNF-a.

Keywords: Eupalitin-3-O-ß-D-galactoside, Conconavalin-A (ConA), Tumor necrosis factor (TNF-a), Nuclear factor-kappaB (NF-?B), P38 MAPKinase

How to cite this article:
: *K. A. Fathima1, C. S.

Introduction   Top

Materials and methods   Top

Results Discussion   Top

Conclusion   Top

Acknowledgement   Top

References   Top

Figures

Immunosuppressive effect of Eupalitin-3-O-?-D-Galactoside in Conconavalin-A induced KOI CARP (Cyprinus Carpio)

K. A. Fathima, C. S. Parameswari

Correspondence Address:1Associate Professor in Biochemistry, Bharathiwomen’s college, Chennai-108, Tamilnadu, India. 2 Associate professor in Biochemistry, Bharathiwomen’s college, Chennai- 108,Tamilnadu,India.

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-4040.197331

Abstract  

Eupalitin-3-O-ß-D-galactoside a bioflavonoid isolated from Boerhaavia diffusa has been reported to possess immunosuppressive activity.To investigate the potential therapeutic effect of Eupalitin-3-O-ß-D-galactoside,Koi carp a fish model was induced with Conconavalin-A and Eupalitin-3-O-ß-D-galactoside was injected intramuscularly 48 hrs after Conconavalin-A administration.The results revealed that Eupalitin-3-O-ß-D-galactoside attenuated the activity of serum lysozyme and myeloperoxidase in serum and expression of TNF-a expression in head kidney tissues of koi carp.Western blotting results revealed that NFkB and p38 MAPK expression were downregulated after Eupalitin-3-O-ß-Dgalactoside treatment.These results suggested the anti-inflammatory effect of Eupalitin-3-O-ß-D-galactoside in Conconavalin-A induced koi carp possibly through inhibition of NFkB and p38 MAPK that mediates the expression of pro inflammatory cytokine TNF-a.

Keywords: Eupalitin-3-O-ß-D-galactoside, Conconavalin-A (ConA), Tumor necrosis factor (TNF-a), Nuclear factor-kappaB (NF-?B), P38 MAPKinase

How to cite this article:
: K. A. Fathima*

Introduction   Top

Materials and methods   Top

Results Discussion   Top

Conclusion   Top

Acknowledgement   Top

References   Top

Figures