Journal of Advanced Pharmacy Education & Research
Year : 2012   |  Volume : 2   |  Issue : 3   |  Page : 93-100  

Development and validation of a HPLC analytical assay method for amlodipine besylate tablets: A Potent Ca+2 channel blocker

Richa Sah and Saahil Arora

Correspondence Address:ISF College of Pharmacy, Moga, Punjab, India

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2231-4040.197331


Amlodipine besylate is a potent calcium channel blocker used for the treatment of hypertension, congestive heart failure and angina pectoris. Amlodipine besylate avoids the adverse effect of amlodipine in racemic mixtures. A highly precise and cost effective RP-HPLC method with retention time of 2.60 minutes was developed for the estimation of amlodipine besylate in tablet dosage form, by fixing the parameters as WATERS C18 column 250 mm × 4.6 mm (5µm), with mobile phase as acetonitrile: 70mM potassium dihydrogen orthophosphate buffer: methanol (15:30:55) and pH adjusted to 3.0 using OPA. Mobile phase flow rate was maintained at 1.0 ml/min and detected at 240nm.

Keywords: RP-HPLC, amlodipine besylate, diode array detector (DAD).

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Materials and methods   Top

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